Archaeologists have found traces of nicotine in flasks from the Late Classic Maya period (600 to 900 AD), which is the first evidence that they used tobacco. The flask is marked with Mayan hieroglyphics reading, “y-otoot ’u-may” (the home of its/his/her tobacco). The researchers used gas chromatography mass spectrometry (GCMS) and liquid chromatography mass spectrometry (LCMS) to find the traces.

Read the abstract in Rapid Communications in Mass Spectrometry.

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